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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were documented but comprehensive nucleotide sequence information and facts is just not however obtainable.

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Blocking peptides are peptides that bind precisely on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.

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This manual outlines further more feasible optimization of cellular and immunoassay parameters to be certain the absolute best final results are...

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This information outlines additional attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide now not bind to your epitope to the concentrate on protein. By evaluating the staining from the blocked antibody compared to the antibody by itself, you can see which staining is precise.

Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates with the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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